Comparison of Root End Sealing Ability of Three Retrograde Filling Materials in Teeth with Root Apices Resected at 90° using Dye Penetration Method under Fluorescent Microscope: An In-vitro Study

Background: Inadequate seal at the apex is substantial cause for surgical endodontic dissatisfaction. The retrograde filling material which used should prevent egress of potential contaminants into periradicular tissue.
 Objectives: To evaluate ability MTA Angelus, Zirconomer and Bioactive bone cement to root end as material; compare sealing these three different materials with apices resected 900 angles using dye penetration method under fluorescent microscope.
 Methodology: Thirty six extracted upper anterior teeth are be cut horizontally CEJ. After following standard protocols of, “cleaning, shaping obturation” gutta percha “AH Plus sealer”, samples will 3mm apical 90 degree angle along long axis tooth help diamond disc. A cavity depth made Diamond coated ultrasonic tip S12 ND. randomly categorized in 3 groups: Group 1: angelus; 2: Zirconomer; 3: Bone Cement. Following roots nail varnish except tip. Each compressed small pluggers. All retrofilled kept acrydine orange a duration 24 hours, cleaning & bucco-lingual sectioning(vertical) done. Fluorescent microscope observation sectioned samples.
 Expected Results: expected have better preparation minimal or no microleakage followed by Angelus then Zirconomer.
 Conclusion: If this study proves correct, would helpful clinicians choose most efficient best aid success canal treatment further resolving infection.